What is the purpose of performing Kato technique?

What is the purpose of performing Kato technique?

The Kato-Katz technique facilitates the detection and quantification of helminth eggs that infected subjects pass in their faeces. A thick smear is prepared on a microscopic slide and helminth eggs are enumerated under a light microscope and recorded for each helminth species separately.

What are the different materials reagents and equipment needed for the Kato-Katz technique?

Materials

• Kato-set (template with hole, screen, nylon or plastic, plastic spatula)
• Newspaper or glazed tile.
• Microscope slides.
• Cellophane as cover slip, soaked in glycerol-malachite green or glycerol-methylene blue solution.
• Fresh stool.
• Gloves.

How do you do the Kato-Katz technique?

The principle behind the Kato-Katz technique is straightforward: people infected with STH or intestinal schistosomes pass the eggs of the worms through their faeces. By examining a stool sample under a microscope it is possible to count the number and the type of eggs that are present.

What is the advantage of using Kato thick technique?

The advantages of Kato-Katz are its low cost, short sample preparation time, simple handling and the need of only basic equipment [8,10]. However, the method has a low sensitivity for low STH infection intensities, hookworm eggs disappear after one hour and samples and slides for hookworm cannot be stored [11–13].

What are the disadvantages of Kato technique?

Disadvantages

• Disadvantages.
• Take time (The Kato-Katz methods require between 1 to 2 hoursbefore the glycerin clears the background of the stoolsmear on the slide for accurate visualization of mosthelminth eggs )

What parasite can best be recovered by Kato Katz method?

Helminthic Diseases: Intestinal Nematode Infections The cellophane fecal thick smear (Kato-Katz technique) for parasite eggs (or larvae in the case of S. stercoralis) remains the best option for diagnosis of STH infections.

What is sedimentation technique?

Sedimentation techniques use solutions of lower specific gravity than the parasitic organisms, thus concentrating the latter in the sediment. It takes advantage of the high specific gravity of protozoan cysts and helminth eggs compared to water. Fecal debris is extracted into the ethyl acetate phase of the solution.

Which of the following will clear rapidly on the Kato-Katz technique?

Hookworm eggs clear rapidly and will no longer be visible after 30-60 minutes. Schistosome eggs may be recognizable for up to several months but it is preferable in a schistosomiasis endemic area to examine the slide preparations within 24 hours.

What are the precautionary measures needed to be practiced in concentration techniques?

These include:

• Wear protective safety glasses, gloves and laboratory coat when processing specimens.
• Use biological safety cabinets as needed.
• Do not eat, drink, smoke, apply cosmetics or manipulate contact lenses in work area.

What is baermann technique?

The Baermann Technique operates on the principle that the nematode larvae wiggle out of the biological material, cannot swim against gravity and will fall through the water to the area of clamped off tubing. The clamp is released to collect the larvae for identification.

What parasite can be best recovered by Kato Katz method?

How do you do a direct fecal smear?

Once a sample is obtained, a direct fecal smear is made by spreading a thin film of feces on a glass slide and adding a few drops of saline. The slide is then examined under a microscope for evidence of microscopic organisms. A fecal smear can also be used to examine fecal cytology—the cells contained in the specimen.

What is the Kato technique and what is its purpose?

Kato technique. Jump to navigation Jump to search. The Kato technique (also called the Kato-Katz technique) is a laboratory method for preparing human stool samples prior to searching for parasite eggs.

How does the Kato Katz technique remove faeces?

In the Kato-Katz technique, faeces are pressed through a mesh screen to remove large particles. A portion of the sieved sample is then transferred to the hole of a template on a slide. After filling the hole, the template is removed and the remaining sample is covered with a piece of cellophane soaked in glycerol.

What do you need to know about Kato Katz?

Kato Katz technique is used for qualitative and semi-quantitative diagnosis of intestinal helminthic infestations; caused by Ascaris lumbricoides, Trichuris trichiura, hookworm and especially Schistosoma spp. WHO has recommended Kato Katz technique in areas with moderate…

What kind of stain is used in Kito Katz?

The stain used in the Kito Katz technique is Glycerol – malachite green or glycerol- methylene blue solution. Composition -1 ml of 3% aqueous malachite green or 3 % methylene blue is added to 100 ml of glycerol and 100 ml of distilled water and mixed well.