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How are serial dilutions used in real life?
Serial dilutions are often used by scientists to quickly and accurately lower the concentration of a liquid. However, making serial dilutions of the well water make it so that the bacteria can be easily counted, like the cereal in the last cup.
What are the two main uses of the serial dilution?
Serial two-fold and ten-fold dilutions are commonly used to titer antibodies or prepare diluted analytes (for a standard curve for example). Serial dilutions are also commonly used to avoid having to pipette very small volumes (1-10 µl) to make a dilution of a solution.
Why are serial dilutions used?
In microbiology, serial dilutions (log dilutions) are used to decrease a bacterial concentration to a required concentration for a specific test method, or to a concentration which is easier to count when plated to an agar plate.
What is the purpose of serial dilutions?
The objective of the serial dilution method is to estimate the concentration (number of colonies, organisms, bacteria, or viruses) of an unknown sample by counting the number of colonies cultured from serial dilutions of the sample, and then back track the measured counts to the unknown concentration.
Are serial dilutions accurate?
The accuracy ratio is an average of the concentration of the diluted column compared to the previous column—a perfect serial dilution has an accuracy ratio of 1:2.00 across the entire plate. The accuracy ratio of the plate improved with more mix cycles, improving from 1:1.85 to 1:2.01.
Why are serial dilutions more accurate?
The more evenly spaced the calibration standards are over this range, makes the results of the analysis more reliable. Each successive standard uses a small portion of the previous standard, which is diluted by solvent to generate the next calibration standard in the series.
How do you explain serial dilution?
A serial dilution is the stepwise dilution of a substance in solution. Usually the dilution factor at each step is constant, resulting in a geometric progression of the concentration in a logarithmic fashion.