What is the difference between viewing a specimen under low power and under high power?

What is the difference between viewing a specimen under low power and under high power?

The field of view is widest on the lowest power objective. When you switch to a higher power, the field of view is closes in. You will see more of an object on low power. The depth of focus is greatest on the lowest power objective.

What happens to the field of view when you switch from LPO to HPO?

Going to high power on a microscope decreases the area of the field of view. The field of view is inversely proportional to the magnification of the objective lens. The specimen appears larger with a higher magnification because a smaller area of the object is spread out to cover the field of view of your eye.

How is the image you view under the microscope different than how the specimen actually looks?

When light reflects off of an object being viewed under the microscope and passes through the lens, it bends towards the eye. This makes the object look bigger than it actually is. Both of these microscopes have an objective lens, which is closer to the object, and an eyepiece, which is the lens you look through.

What is the function of the LPO on a microscope?

Low power objectives cover a wide field of view and they are useful for examining large specimens or surveying many smaller specimens. This objective is useful for aligning the microscope. The power for the low objective is 10X. Place one of the prepared slides onto the stage of your microscope.

Why does the specimen must be centered?

You must have the object centered before you change objectives to increase the magnification, because the field of view becomes smaller; if the object is off to the side, it may disappear when you go to higher magnification. For best viewing at high power, white light is essential.

Which level of magnification requires the most illumination?

45x requires the most illumination for best clarity and contrast. Adjusting the light intensity is important to see a more clear view of the specimen.

What is the total magnification of LPO HPO and OIO?

Write your answer… What is the magnification of the specimen under lpo and hpo? The magnification of the specimen under low power optics, lpo, is 10 times and the magnification of the specimen under high power optics, hpo, depends on the power of the microscope but is usually at least 500 times or more.

Why do you need to focus specimens under LPO and HPO?

It appears to get bigger but, is still blurry. Why do you need to focus specimens under the LPO first before focusing under the HPO? It allows you to center the specimen, and if it is set up correctly, then when you switch objectives it should still be there and only need fine focusing.

What is the function of the LPO in a microscope?

It allows you to center the specimen, and if it is set up correctly, then when you switch objectives it should still be there and only need fine focusing. It also reduces the chance of crushing the specimen and/or breaking the slide or the lens as the LPO is further away from the slide than the HPO. Function of LPO in microscope?

What does HPO stand for in a microscope?

The Word “HPO” in Microscope stands for High Power Objective.Most standard compound microscopes use a combination of an eye piece lens and objective lenses in order to magnify an object.